Highly Purified Colicin E3 Contains Immunity Protein
نویسندگان
چکیده
منابع مشابه
The three-dimensional structure of the colicin E3 immunity protein by distance geometry calculation.
The three-dimensional solution structure of the colicin E3 immunity protein (84 residues) was determined by distance geometry calculations. The hydrophilic side of a four-stranded antiparallel beta-sheet constitutes a part of the surface of the protein, and two loops lie on the hydrophobic side of the sheet. All the three specificity-determining residues, which are included in the center of the...
متن کاملAssociation and dissociation kinetics of colicin E3 and immunity protein 3: convergence of theory and experiment.
The rapid binding of cytotoxic colicin E3 by its cognate immunity protein Im3 is essential in safeguarding the producing cell. The X-ray structure of the E3/Im3 complex shows that the Im3 molecule interfaces with both the C-terminal ribonuclease (RNase) domain and the N-terminal translocation domain of E3. The association and dissociation rates of the RNase domain and Im3 show drastically diffe...
متن کاملThe purified colicin S8 is a multimeric protein.
Bacteriocins have been isolated both as simple proteins and as proteins in association with carbohydrates, lipids, etc. Colicins are commonly inducible and extracellular. Their molecular masses range from 30 to 90 kDa. Pure colicin S8 was obtained in three steps from supernatant of induced cells: (i) Ammonium sulfate precipitation; (ii) anion exchange chromatography; and (iii) phenyl-Sepharose ...
متن کاملRelease of immunity protein requires functional endonuclease colicin import machinery.
Bacteria producing endonuclease colicins are protected against the cytotoxic activity by a small immunity protein that binds with high affinity and specificity to inactivate the endonuclease. This complex is released into the extracellular medium, and the immunity protein is jettisoned upon binding of the complex to susceptible cells. However, it is not known how and at what stage during infect...
متن کاملIn vitro synthesis of the E3 immunity protein directed by Col E3 plasmid deoxyribonucleic acid.
E3 colicinogenic cells are immune to colicin E3. A protein called "E3 immunity protein" was previously isolated from E3 colicinogenic cells and shown to prevent the E3-induced in vitro inactivation of ribosomes. We now show that the structural gene for E3 immunity protein resides on the Col E3 plasmid, a plasmid which is present in E3 colicinogenic cells and carries the structural gene for coli...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1974
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.71.9.3380